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pcr  (New England Biolabs)


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    Structured Review

    New England Biolabs pcr
    Pcr, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptyb21/pTYB21+Vector/pm41714452-258-5-10
    Average 94 stars, based on 87 article reviews
    pcr - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis
    Article Snippet: The vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sap I and EcoR I (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Agarose Gel Electrophoresis:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis
    Article Snippet: The vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sap I and EcoR I (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Gel Extraction:

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis
    Article Snippet: The vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sap I and EcoR I (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Article Title: Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.
    Article Snippet: The TABLE 2 Rhodococcus equi strains used in this study Strain name Source vap gene Description Reference ATCC33701 A pneumonic foal vapA VapA-producing strain (34) U19 Tracheal lavage fluid of a Friesian horse vapA VapA-producing strain (35) S4 Mandibular lymph node of a pig vapB VapB-producing strain (36, 37) JCM94-3 AIDS patients in Switzerland vapN VapN-producing strain (14, 15) JID03-27 AIDS patients in Thailand vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-25 Renal transplant patients in the USA vapN VapN-producing strain (14, 15) JCM94-27 Lymphosarcoma patients in New Zealand vapN VapN-producing strain (14, 15) JCM94-14 AIDS patients in Australia vapN Weakly VapN-producing strain (14, 15) JCM94-16 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) JCM94-31 AIDS patients in the USA vapN Strain carrying the VapN gene but not producing VapN protein (14, 15) November/December 2023 Volume 11 Issue 6 10.1128/spectrum.00729-23 11 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 14 J an ua ry 2 02 4 by 1 14 .4 .2 14 .1 75 . vapN fragment was amplified from gDNA by PCR using Tks Gflex DNA Polymerase (Takara Bio, Shiga, Japan). .. Another DNA fragment was prepared by sapI and EcoRI (New England Biolabs) digestion of pTYB21 (New England Biolabs) and purification after agarose gel electrophoresis using a QIAquick Gel Extraction Kit (Qiagen GmbH). pTYB21 is an E. coli expression system of the target protein to be fused to the intein tag. ..

    Polymerase Chain Reaction:

    Article Title: Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.
    Article Snippet: .. To produce pTYB21-PsTyDC1, pTYB21-PsPDC1, pTYB21-PsPDC2 and pTYB21-Ps2HCLL, PsTyDC1, PsPDC1, Ps2HCLL, and N-terminal truncated PsPDC2 were PCR amplified and cloned into pTYB21 digested with SapI and BamHI via Gibson assembly (NEB)63. ..

    Article Title: Differential regulation of Shigella Spa47 ATPase activity by a native C-terminal product of Spa33.
    Article Snippet: Escherichia coli strains and 2× ligation mix were from Novagen (Madison, WI). .. Restriction enzymes, the commercial pTYB21, pACYC, pRSF, and pET15b protein expression plasmids, polymerase chain reaction (PCR) buffer, Q5 High-Fidelity polymerase, and chitin resin were purchased from New England Biolabs (Ipswich, MA). .. Oligonucleotide primers were from Integrated DNA Technologies (Coralville, IA).

    Article Title: Differential regulation of Shigella Spa47 ATPase activity by a native C-terminal product of Spa33
    Article Snippet: Escherichia coli strains and 2× ligation mix were from Novagen (Madison, WI). .. Restriction enzymes, the commercial pTYB21, pACYC, pRSF, and pET15b protein expression plasmids, polymerase chain reaction (PCR) buffer, Q5 High-Fidelity polymerase, and chitin resin were purchased from New England Biolabs (Ipswich, MA). .. Oligonucleotide primers were from Integrated DNA Technologies (Coralville, IA).

    Amplification:

    Article Title: Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.
    Article Snippet: .. To produce pTYB21-PsTyDC1, pTYB21-PsPDC1, pTYB21-PsPDC2 and pTYB21-Ps2HCLL, PsTyDC1, PsPDC1, Ps2HCLL, and N-terminal truncated PsPDC2 were PCR amplified and cloned into pTYB21 digested with SapI and BamHI via Gibson assembly (NEB)63. ..

    Clone Assay:

    Article Title: Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.
    Article Snippet: .. To produce pTYB21-PsTyDC1, pTYB21-PsPDC1, pTYB21-PsPDC2 and pTYB21-Ps2HCLL, PsTyDC1, PsPDC1, Ps2HCLL, and N-terminal truncated PsPDC2 were PCR amplified and cloned into pTYB21 digested with SapI and BamHI via Gibson assembly (NEB)63. ..

    Article Title: Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.
    Article Snippet: Native coding sequences of fulllength EcNMCH, AtATR2, and P. somniferum CPR-like (PsCPR-L) were synthesized and subcloned into the pMA vector by GeneArt (Invitrogen). .. Native coding sequences of full-length PsTyDC6 and N-terminal truncated PsPDC1-IX1 were synthesized and cloned into pTYB21 (NEB) by GenScript (Piscataway, NJ, USA). ..

    Synthesized:

    Article Title: Machine learning discovery of missing links that mediate alternative branches to plant alkaloids.
    Article Snippet: Native coding sequences of fulllength EcNMCH, AtATR2, and P. somniferum CPR-like (PsCPR-L) were synthesized and subcloned into the pMA vector by GeneArt (Invitrogen). .. Native coding sequences of full-length PsTyDC6 and N-terminal truncated PsPDC1-IX1 were synthesized and cloned into pTYB21 (NEB) by GenScript (Piscataway, NJ, USA). ..

    Expressing:

    Article Title: Differential regulation of Shigella Spa47 ATPase activity by a native C-terminal product of Spa33.
    Article Snippet: Escherichia coli strains and 2× ligation mix were from Novagen (Madison, WI). .. Restriction enzymes, the commercial pTYB21, pACYC, pRSF, and pET15b protein expression plasmids, polymerase chain reaction (PCR) buffer, Q5 High-Fidelity polymerase, and chitin resin were purchased from New England Biolabs (Ipswich, MA). .. Oligonucleotide primers were from Integrated DNA Technologies (Coralville, IA).

    Article Title: Differential regulation of Shigella Spa47 ATPase activity by a native C-terminal product of Spa33
    Article Snippet: Escherichia coli strains and 2× ligation mix were from Novagen (Madison, WI). .. Restriction enzymes, the commercial pTYB21, pACYC, pRSF, and pET15b protein expression plasmids, polymerase chain reaction (PCR) buffer, Q5 High-Fidelity polymerase, and chitin resin were purchased from New England Biolabs (Ipswich, MA). .. Oligonucleotide primers were from Integrated DNA Technologies (Coralville, IA).



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